Advanced Real-time PCR Training

This one-day advanced real-time PCR course focuses on the critical steps of gene expression studies, such as normalisation, validation and statistical analysis.

1 day
Course fee
€ 208 (excl. VAT)

Short description

In this course the correct use and practice of relative quantification software and of secondary analysis will be explained. Also methods to optimise the use of instruments and consumables will be explored.

The course is intended for all experienced users of real-time PCR that are interested in gene expression application and want to have more knowledge of all aspects of the gene expression workflow. Course participants will gain an insight into the appropriate experimental setup and analysis of gene expression results.

The outline of this interactive course will range from good sample preparation, customer primer and/or assay design, real-time PCR experiment setup and biostatistics including data analysis with primary and secondary cloud-based software packages.

Target group

The course is intended for all experienced users of real-time PCR who want to obtain more in-depth understanding of mRNA, miRNA, ncRNA expression analysis experiments, e.g. scientists, biological and medical technicians. 

It is recommended that participants in this training have attended the basic real-time PCR course or have corresponding knowledge of real-time PCR.


Day 1


By combining theoretical training and practical software workshops, the following topics are covered:
• Introduction and review of qPCR
• Experimental planning, validation and normalisation
• Biostatistics: A review and short introduction
• qPCR and bioinformatics
• Analysing and reporting qPCR results
• Multiplex qPCR
• Optimisation and troubleshooting

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Interested in signing up a group of people for this course?

Please contact our Business Development team:
+31 (0)88 2830100

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Drop us a line below or call us on
+31 (0) 88 283 01 00.