Introduction to HPLC (level 1)

This course offers you all the practical information necessary when it comes to the basic principles of HPLC, including the various components of instruments and the different types of columns used in HPLC.

3 days
Course fee
€ 1530 (excl. VAT)

Short description

The course will not only provide you with theoretical information but will also give you ample opportunity for practical training with HPLC instruments in our laboratory.

All the information provided by this course is directly applicable to the daily conditions of HPLC analyses within your own laboratory environment. Yes, you will learn theoretical applications throughout this course but you will also actually use the various types of HPLC instruments present in our laboratory. Classical teaching methods and laboratory training will alternate constantly. For the practical training you will be divided into small groups and carry out experiments under expert supervision, using  only the most modern equipment available. Each group will switch instrumentation frequently so that you can familiarize yourself with the various types of equipment.

This course is realized in partnership with Avans+.


  • Be able to start up an HPLC system on your own.
  • Be able to use a test chromatogram to determine the status and quality of the system.
  • Obtain a good overview of the HPLC components, the properties of the types of injectors and detectors, the mobile phases and the most common of the HPLC columns.

Target group

You have not been working long with HPLC and you would very much like to improve your HPLC knowledge and skills necessary for your daily activities. You want to get a better understanding of HPLC separation and columns, mobile phases, quantitative analysis and associated equipment. The course is meant for all laboratory staff who use HPLC to analyze the most diverse of samples.


Day 1


+ Introduction to HPLC

Terminology of the modern HPLC, principles of the separation process, retention, resolving power, selectivity, resolution and peak asymmetry. Determine the framework conditions to achieve a good HPLC separation.


+ Instrumentation for HPLC

We deal with liquid reservoirs, filters, degassing of mobile phases, types of pumps, gradient pump systems, injection valves, fittings, columns, detectors and data processing.

Day 2


+ Qualitative and quantitative analysis

We also examine peak identification, sample preparation, detector response, calibration, external standards, internal standards, standard addition and the normalized 100% method.


+ HPLC techniques

You will be acquainted with phase types and separation mechanisms, chromatographic systems, adsorption chromatography, reversed phase chromatography, chemical bonded phases, polar bonded phases, ion exchange chromatography (IC) and size exclusion chromatography (SEC).

Day 3


+ Reversed phase liquid chromatography (RPLC)

After a short introduction we will discuss the application of RPLC, RP stationary fase material, the influence of the stationary phase on separation, mobile phase for RPLC, variations and selectivity of the organic modifiers and the influence of the mobile phase on retention.


+ Isocratic elution vs gradient elution

We refer to a description of the liquid gradient and the practical aspects of the equipment. We discuss the pros and cons of gradient elution, high and low pressure gradient mixing, pump system volumes and the importance of the purity of the solvents.

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Interested in signing up a group of people for this course?

Please contact our Business Development team:
+31 (0)88 2830100

Know how.
Know why.

Need help? Have a question or a suggestion maybe?

Drop us a line below or call us on
+31 (0) 88 283 01 00.