Introduction to Proteomics
Are you interested in research methods in proteins and ready to put your gloves on? This course offers basic preparative and analytical techniques for proteins, more specifically, extraction, quantification, concentration, and expression analysis.
- 2 days
- Course fee
- € 1495 (excl. VAT)
Proteins are the structural and functional blocks of living organisms. Analyzing proteins is very important to understand how cellular processes work and how changes in genetic level affect protein expression.
Proteins can be found in various locations in an organism, such as in blood, in cellular matrix, and on the cell membrane. Based on their locations, a suitable extraction and homogenization method needs to be selected. After a careful extraction, the concentration of proteins is determined by several methods and if needed the concentration of proteins can be increased physically or chemically.
Different conditions such as diseases, medical treatments and living conditions can alter the amount and repertoire of proteins in a specific time and place. Analyzing these alterations in protein level helps researchers to find out molecular background of diseases, possible treatment methods, and also to identify novel proteins. In order to observe changes in protein levels several methods such as SDS-PAGE, 2D Gel Electrophoresis, and Western Blotting are used.
This course offers the fundamentals of research methods in protein studies. You will gain insight about several techniques in proteomic research and first-hand experience on those techniques.
- To acknowledge the importance of proteomic research
- To gain experience in research methods in protein studies
- To be familiar with the requirements and conditions of research processes in proteomics
Researchers, laboratory personnel, technicians and anyone with an interest in molecular biology.
Theory: Introduction to proteomics
Background information on proteins. History of proteomic research. Workflow in protein studies. Preparative processes (homogenization, extraction, concentration determination) and analytical processes (SDS-PAGE, 2D Gel Electrophoresis, Western Blotting, Mass Spectrometry). Critical points while working with proteins. Recent techniques in proteomics. Future of proteomics.
Practice: Buffers and homogenization
Protein extraction from different sources (animal cell, plant cell, microorganisms) with different homogenization techniques (manual grinding, mechanical disruption). Importance of using buffers (Tris buffer, phosphate buffer etc.).
Practice: Concentration Determination
Determining the concentration of proteins from different sources with widely-used methods (UV absorbance, Bradford method, Lowry method, BCA protein assay).
Practice: Preparation for SDS-PAGE
Polyacrylamide gel preparation. Denaturation (chemically and physically) of samples for loading into polyacrylamide gels.
Loading and running the polyacrylamide gels.
Practice: Analysis of results
Visualization of polyacrylamide gels. Markers for size determination. Interpretation of results.
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+31 (0) 88 283 01 00.