Introduction to Upstream Processing

What is Upstream Processing? Learn all about the basic principles of upstream processing in biotechnology with this practical training course. And practice the upstream processing techniques yourself in a realistic production environment.

1 day
Course fee
€ 995 (excl. VAT)

Short description

What can you expect from this Upstream Processing training course?

Average score from participants of the previous course: 9!

This training course provides all background information needed to fully understand the Upstream Processing part of the production of biopharmaceutical products in a GMP environment.

The theory section explains the basis of various aspects that are essential for the Upstream Processing part of production of biopharmaceutical products in a interactive way, with topics related to cell culturing, in process controls and an introduction to P&ID. 

The practical part of this course takes place in a real production environment, allowing the participants to perform various techniques (thawing cells, cell count and an interactive part with a bioreactor) of Upstream Processing themselves and in addition experience the importance of working in a cleanroom. 

This course can be followed in combination with the training course Introduction into Downstream Processing (1 day)


After the training course, the participants have knowledge of:

  • the different process steps that are important for the Upstream Proces 
  • basic principles of cell culture
  • basic principles of the propagation of viruses
  • construction and maintenance of a cell bank/seed lot
  • Practical experience in aseptic handling

  • Practical experience in automatic cell counting

Target group

Who should follow this Upstream training course? 

  • Anyone who wants to know more about the upstream processing of biopharmaceutical production. These may include for instance production operators, QA and auditors.
  • This course is also intended for QC analysts and validation staff who want to increase their practical knowledge about upstream production of biotechnological products.

Not the training course you were looking for?

See our other courses on biopharmaceutical production processes:


Day 1



Biopharmaceutical production & upstream processing
The production process of common biotechnological products such as antibodies, viruses and pharmaceutical proteins
Pharmaceutical microbiology
How we can prevent microorganisms from ending up in pharmaceutical products
The functioning of cleanrooms



Cleanroom principles - upstream processing
Various techniques needed to grow bacterial and mammalian cells, including:
• Cell bank principles
• Defrost and freeze
• Required process parameters for cell culture and virus multiplication
• Challenges in cell culture and virus multiplication

The above techniques are carried out in a practical, real-life environment, where certain aspects are performed by the participants themselves and other aspects are shown and explained in a demonstration.



Sign up

Interested in signing up a group of people for this course?

Please contact our Business Development team:
+31 (0)88 2830100

Need help? Have a question or a suggestion maybe?

Drop us a line below or call us on
+31 (0) 88 283 01 00.