Real-Time PCR Training Course - Theory and Optimization

May 15, 2019

The aim of this one-day course is to get familiar with the theory and practice of real-time PCR and to learn the most cost-effective way to set up, run and analyse experiments. Participants will also have the opportunity to discuss their real-time PCR experiments with application support specialists, and to discover new real-time PCR development methods.

Course Content

  • Principles of and workflows using real-time PCR
  • Applications for quantitative and qualitative analysis
  • Absolute and relative quantification
  • Sample preparation: choice of the correct extraction/purification method
  • Assay design: from sequence to primer and probe
  • Measuring assay efficiency
  • Basic troubleshooting

Target group

This course is intended for all experienced users of conventional PCR who plan to start with real-time PCR, as well as users who would like to learn more about background, applications and optimisation e.g. scientists, PhD students, biological and medical technicians.

Your trainer: Thermo Fisher Scientific

Our training team consists of highly qualified staff members, who have well-founded experience in professional support for real-time PCR.

Course fee: €208

This course will take place at 2 different venues:

4 June 2019 - Venue:

Park Inn Hotel Brussels Midi

Place Marcel Broodthaers 3

(Opposite Brussels Midi railway station)

1060, Bruxelles - Belgium

18 June 2019 - Venue:

Biotech Training Facility

Sylviusweg 70

2333 BE Leiden (Bio Science Park)

For more info and to register for one of the limited training places, please click here